• The demand by the Congress for an NIA probe against Assam's CM and Ram Madhav is significant because it underlines the political tensions between the two parties in the state.
• The National Investigation Agency or NIA is a federal agency that deals with cases related to national security and terrorism. Therefore, allegations against individuals by the opposition party can have serious consequences.
• The specific allegations against the Assam CM and Ram Madhav relate to their alleged involvement in a conspiracy to disturb peace and stability in the state.
• The Congress claims that the Assam CM and Ram Madhav have been trying to instigate communal tensions in the state, especially in the run-up to the state elections later this year.
• An NIA probe would be significant because it would signal the involvement of a federal agency, thereby lending credibility to the allegations made by the Congress.
• At the same time, an NIA probe could also be seen as politically motivated by the BJP, which is currently in power at the center.

In summary, the demand for an NIA probe against Assam's CM and Ram Madhav by the Congress is significant because it underlines the political tensions in the state, and the involvement of a federal agency could lend credibility to the allegations made by the opposition party. However, an NIA probe could also be seen as politically motivated by the ruling party.