The impact of the New Assisted Reproductive Technology (ART) regulations on the cost of treatment and conception opportunities in India can be examined as follows: 1. Cost of treatment: The new regulations have led to an increase in the cost of ART treatment in India. The stricter guidelines, such as mandatory registration and accreditation of clinics, have increased operational expenses and, consequently, the cost borne by patients. Additionally, the requirement for single embryo transfer (SET) to reduce multiple pregnancies has resulted in fewer chances of success per cycle, leading to higher treatment costs. 2. Limit on conception opportunities: The new regulations have imposed restrictions on the number of embryos that can be created and stored per cycle. This has reduced the likelihood of multiple attempts using previously frozen embryos, limiting conception opportunities for patients. Furthermore, the guidelines also prohibit surrogacy for foreign nationals, limiting the options available for those seeking to grow their families through this method. Overall, while the new ART regulations aim to maintain ethical standards and ensure patient safety, they have increased the cost of treatment and limited conception opportunities for individuals in India.